Journal: Molecular Biology of the Cell

Article Title: Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin

doi: 10.1091/mbc.e14-05-0978

Figure Lengend Snippet: FIGURE 4: GIV’s SH2 and GEF domains are required for the recruitment of Gαi3 to ligand- activated EGFR and activation of G protein after EGF stimulation. (a) Equal aliquots (15 μg) of GST-EGFR-T (aa 1064–1210) were either phosphorylated in vitro using 5 ng of recombinant EGFR kinase (GST-pY EGFR-T; lanes 5–7) or mock treated (GST-EGFR-T; lane 4) and subsequently used in pull-down assays with equal amounts of purified WT, FA (GEF- deficient), or RL (SH2-deficient) mutants of His-GIV-CT (aa 1660–1870; lanes 1–3, inputs). Bound proteins were analyzed for His-GIV-CT and phosphorylation of GST-EGFR-T by IB. As anticipated, GST-pY EGFR-T (lane 5) but not GST-EGFR-T (lane 4) directly binds His-GIV-CT

Article Snippet: Rabbit polyclonal antibodies against FLAG (Invitrogen; for immuno- blotting), GST (Santa Cruz Biotechnology), phospho-Y992 EGFR (Cell Signaling), total EGFR (Santa Cruz Biotechnology), Gαi3 (M-14; Santa Cruz Biotechnology), phospho-Akt S473 (Cell Signaling), pan-Gβ (Santa Cruz Biotechnology), and phospho-ERK 1/2 (Cell Signaling) were obtained commercially.

Techniques: Activation Assay, In Vitro, Recombinant, Purification, Phospho-proteomics

Journal: iScience

Article Title: Encephalopathy-causing mutations in Gβ 1 ( GNB1 ) alter regulation of neuronal GIRK channels

doi: 10.1016/j.isci.2021.103018

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Gβ (T-20) (1:500) , Santa Cruz Biotechnology , sc-378; RRID: AB_631542 ; currently discontinued.

Techniques: Recombinant, Saline, Modification, Mutagenesis, Software